Re: HELP: HOW TO DOWNLOAD THE TOTAL PROTEIN SEQUENCE DATABASE OF MOUSE FROM NCBI




"Ron O" <rokimoto@xxxxxxx> wrote
>> When I try to use LTQ to analyze the protein of mouse liver, I find
>> that the sequence of protein is one protein and another, if I down them
>> one by one, perhpas one year is needed, how can I down the total
>> protein sequence of mouse quickly?
>
> Contact the NCBI. They will send you the entire database, but you will
> probably have to pay a fee. If you have a fast connection there may
> also be a means to download parts like the mouse protein database, but
> I've never heard of anyone just taking a piece. It used to be common
> for universities to have Genbank on a local mainframe, but the access
> to supercomputers on the web make it redundant except for constant
> grinding searching.
>
It is not hard to write a perl script to hit public databases. You can also
filter for proteins expressed in the liver (presumbly you have a list, if
not it may be tagged). However if you don't know what you are doing it is
best to ask someone who does - Leeds University had access to one of the
public databases suspended for a few days because someone was tying up their
servers with automated requests. This sort of thing doesn't make you
popular.



.



Relevant Pages

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