Re: molar absorptivity
From: Uncle Al (UncleAl0_at_hate.spam.net)
Date: 10/30/04
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Date: Sat, 30 Oct 2004 16:06:56 -0700
serunato wrote:
>
> I am having a problem locating the molar absorptivity coefficient for
> bromocresol purple. Also known as the extinction coefficient for the
> old school. If anyone can tell me where to find such info I would
> appreciate it. So far I have checked ccr, merck, scifinder,
> e-journals and even my local chemistry librarian who was stumped. and
> I have come up with nill. Any help would be great, thanks.
The lambda(max) and epsilon shift with concentration (dimer
formation), pH; and presence of other species, membranes, and micelles
that participate in non-bonded interactions (re cornflower blue and
caffeic acid). The only way to really know is to measure it in your
system with your indicator (that is *never* pure and varies from lot
to lot).
Maybe
Applied Biochemistry and Microbiology 37(2) 168-173 (2001)
FEBS Letters 503 (2-3) 142 (2001)
SPIE Proceedings Vol. 1711 pp.293-302 "High-Performance Optical
Spectrometry" ISBN: 0-8194-0844-1, 388 pages Published 1993 Meeting
Date: 06/01 - 06/05/92, Warsaw, Poland.
-- Uncle Al http://www.mazepath.com/uncleal/ (Toxic URL! Unsafe for children and most mammals) http://www.mazepath.com/uncleal/qz.pdf
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