Re: Spectrophotometer Help

From: Farooq W (farooq.w_at_gmail.com)
Date: 03/29/05


Date: 28 Mar 2005 18:36:48 -0800


Rosnilda@gmail.com wrote:
> Hello Dan
> I write that I look that maybe interfere in your analisys.
>
> 1. Permanganate is violet and absorb in yellow-geen
> 2. Permanganate need protection from the ligth.
> 3. The absorbance is not more than 2 as law.

This is apparently a misconception: The range of transmittance is 0 to
1 (or say 0 to 100%). Taking negative logarithm of T=1 gives you an
absorbance = 0, when you take negative log of T=0, what do you get?
Infinity. Absorbance values can theoretically range from 0 to infinity.

> 4. For the custom of your graphic it grow up and up and then go down,
> that said that maybe the lectures are very high and the equipment
don't
> have the option to read it or have interferences that bond the
absorbet
> composite.
> 5. I think as you that you have a problem of concentration.
> 6. An strategy is made some dilutions and see about 400nm if read the
> concentration.



Relevant Pages

  • Re: Spectrophotometer Help
    ... > spectophotometer to measure the absorbance of certain solutions. ... Permanganate is purple. ... transmittance is 1.9 absorbance? ...
    (sci.chem)
  • Re: Spectrophotometer Help
    ... : This is apparently a misconception: The range of transmittance is 0 to ... Absorbance values can theoretically range from 0 to infinity. ... and not those of Bar-Ilan University ...
    (sci.chem)