Re: Acetone + ccHCl yields chlorine?

In article <1191880910.315037.94970@xxxxxxxxxxxxxxxxxxxxxxxxxxxx>, WS <novalidaddress@xxxxxxxxxxxxxx> wrote:
Dear Experts,

I have prepared acidic acetone by mixing 95% (v) acetone and 5% (v)
conc. HCl in order to precipitate proteins from serum. After a few
minutes. the solution turned yellowish green (typical chlorine color),
what I didn't expect. There seems to be a weak smell of chlorine as
well. When I add up to 500ul (I haven't tried more) to 50ul serum,
then the green color vanishes quickly.

Can anyone explain what has happened there? acetone is 99.8% pure and
pharmaceutical quality and HCl is p.a (means appox. ACS grade). I
don't think acetone itself cold oxidize Cl- to elementary chlorine.
Could some impurities be the cause?

Thanks for your suggestions!

I don't know - sorry ... but is there any particular reason for using those
chemicals to precipitate protein from serum ? It's kind of an odd choice I
think. I've been doing this sort of thing for 30 ish years now and this is
the first time I've seen that mix. :)

What exactly are you trying to achieve ?


.

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