Re: Acetone + ccHCl yields chlorine?

On Oct 9, 1:03 am, bruce.sincl...@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
(Bruce Sinclair) wrote:
In article <1191880910.315037.94...@xxxxxxxxxxxxxxxxxxxxxxxxxxxx>, WS <novalidaddr...@xxxxxxxxxxxxxx> wrote:

Dear Experts,

I have prepared acidic acetone by mixing 95% (v) acetone and 5% (v)
conc. HCl in order to precipitate proteins from serum. After a few
minutes. the solution turned yellowish green (typical chlorine color),
what I didn't expect. There seems to be a weak smell of chlorine as
well. When I add up to 500ul (I haven't tried more) to 50ul serum,
Hi Bruce,

actually, I had planned to use 10% (m/v) trichloroacetic acid (TCA) in
acetone according to a procedure described by Chen et al. (http://
www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=15880626)
to fractionate serum. As I didn't have TCA on the shelf, I tried the
mentioned mixture as the author compared it to and had found it worked
similar.

Wo

I don't know - sorry ... but is there any particular reason for using those
chemicals to precipitate protein from serum ? It's kind of an odd choice I
think. I've been doing this sort of thing for 30 ish years now and this is
the first time I've seen that mix. :)

What exactly are you trying to achieve ?


.

Relevant Pages

  • Re: Acetone + ccHCl yields chlorine?
    ... HCl in order to precipitate proteins from serum. ... the solution turned yellowish green (typical chlorine color), ... As I didn't have TCA on the shelf, ...
    (sci.chem)
  • Re: Acetone + ccHCl yields chlorine?
    ... HCl in order to precipitate proteins from serum. ... There seems to be a weak smell of chlorine as ...
    (sci.chem)