Re: mass spec analysis help --------------
- From: "kiloVolts" <spamtrap@xxxxxxxxxx>
- Date: Mon, 15 Oct 2007 16:50:00 GMT
Is it a protein or nucleotide? Please give molecular weight of unlabeled
sample. What is molecular formula, CmHnOlNkSq? Tell us something about the
mass spec. Is it ESI, or MALDI? If it was ESI then was it LC/MS. Tell us
somthing about chromatogram. Tell us something about the deconvolution of
multiply charged peaks. This sample is for NMR right? Tell us something
about hetcor.
"Kadi" <skarthickbabu@xxxxxxxxx> wrote in message
news:1192452630.038245.285260@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dear all ,
Is there any better way to find out how much of 13C and 15N labelled
in my sample by mass spec.
current mass spectrum of my sample when comapred to unlabelled sample
shows that it has been labelled by both 13C and 15N.
but i wondering how could i calculate to know how much of each
nuclei
would be labelled.
my sample is isolated from bacteria, so it not like chemical
synthesis , where i could be sure of 100 percent conversion etc.
I think you may be making a false assumption here.
if any software could simulate the spectra , plz leme know so that i
can simulate the spectra for 13C and 15 labeling pattern seaparetley
and compare them to get some rough idea.
will be very happy if some one could help me out.
thanks in advance.
doei
kadi
.
- References:
- mass spec analysis help --------------
- From: Kadi
- mass spec analysis help --------------
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