Re: Merging many microscopic images taken from optical microscope



I have used autostitch for a long time now, awesome piece of software.
However, do inspect the results in detail later.. It uses spherical harmonics and at times distorts the stitched image.

Depending on what you are trying to do with the final image
(pretty picture for a poster.. or as an input to a IP pipeline), be wary.

On Fri, 14 Mar 2008 05:11:23 -0400, Emos <e.osimo@xxxxxxxxx> wrote:

On 10 Mar, 01:24, ImageAnalyst <imageanal...@xxxxxxxxxxxxxx> wrote:
On Mar 9, 5:29 pm, Noone <no...@xxxxxxxxxxx> wrote:



> Emos wrote:
> > Hello everyone,
> > Im' new in this group.
> > I'd like to know if you know a practical way to merge many images
> > (40+) in an unique one, possibly in an automated way.
> > My problem is that I have an optical trinocular microscope to which I
> > attached my Canon EOS camera, and took 40+ images of a slide to cover
> > the entire object, but then I'm unable to merge them in a simple way
> > to form a unique photo.
> > Can you help me?
> > Thank you,

> > Emanuele (Italy)

> What do u mean exactly merge? You mean align them and blend them?
> make a panoramic stitch?
> make a 3d object?

> you need to define what you mean by "merge" and "cover the entire object"

> --
> Posted via a free Usenet account fromhttp://www.teranews.com

---------------------------------------------
Noone:
By covering the entire object, he means that he took a bunch of images
(40 of them), moving the microscope slide nearly one field of view
each time. Each snapshot is roughly 1/40th of the total field of view
if they were all stitched together. There is some overlap of the
images and this will help the stitching programs. By "merge" he means
stitching or "panoramic stitching" as you called it. He didn't say
anything about 3D so I doubt that is of concern to him. He needs to
look for stitching programs. I don't have recommendations for him as
I haven't used any. Has anyone used any good ones that they could
recommend?
Regards,
ImageAnalyst


Thank you, ImageAnalyst, that's exactly what I meant.
I found a computer program that is fantastic for this purpose:
http://www.cs.ubc.ca/~mbrown/autostitch/autostitch.html

Thank you everyone!
Emanuele

.



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