2004: lipoproteins of Bb vary in their ability to induce experimental joint injury.
- From: "CaliforniaLyme" <CaliforniaLyme@xxxxxx>
- Date: 28 Apr 2005 09:02:03 -0700
1: Arthritis Rheum. 2004 Jul;50(7):2360-9. Related Articles, Links
Outer surface lipoproteins of Borrelia burgdorferi vary in their
ability to induce experimental joint injury.
Batsford S, Dunn J, Mihatsch M.
Albert Ludwigs University, Freiburg, Germany. bats@xxxxxxxxxxxxxxxxxxx
OBJECTIVE: To examine the ability of bacterial lipoproteins from the
spirochete Borrelia burgdorferi to cause in vivo tissue injury
(arthritis). METHODS: Outer surface proteins (OSPs) from B burgdorferi
were used in a rat model of antigen-induced allergic arthritis.
Intraarticular challenge with recombinant OspA, OspB, and OspC in
nonlipidated (peptide) and lipidated forms was performed in the left
knee joint; the contralateral joint received buffer as control.
Inflammation was monitored by technetium scintigraphy and histology.
RESULTS: Nonlipidated (peptide) OspA, OspB, and OspC did not induce
arthritis; the only exception was polymerized OspA, which was tested in
preimmunized rats. Lipidated OspA from 2 different strains and
lipidated OspC induced severe arthritis, whereas lipidated OspB failed
to induce injury. A synthetic analog of the OSP lipid modification,
lipopeptide Pam(3)Cys-Ser-Lys(4)-OH, either alone or coupled to bovine
serum albumin, also failed to induce injury. Injury did not develop in
control groups that were given the appropriate buffers or
lipopolysaccharide. This showed that lipidated borrelial OSPs can be
potent arthritogens but vary greatly with respect to their
injury-inducing potential. The possession of a lipid modification is
essential but is not sufficient to render an OSP arthritogenic.
CONCLUSION: This is the first study to demonstrate that individual
lipoproteins from B burgdorferi can induce experimental joint injury in
vivo. These results may help elucidate the pathogenesis of Lyme
arthritis and, above all, underline the importance of bacterial
lipoproteins as major virulence factors.
PMID: 15248237 [PubMed - indexed for MEDLINE]
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