2005: Detection of Immune Complexes Does Not Confirm Active Infection with Bb
- From: "CaliforniaLyme" <CaliforniaLyme@xxxxxx>
- Date: 9 Sep 2005 08:35:43 -0700
1: Clin Diagn Lab Immunol. 2005 Sep;12(9):1036-40. Related Articles,
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Detection of Immune Complexes Is Not Independent of Detection of
Antibodies in Lyme Disease Patients and Does Not Confirm Active
Infection with Borrelia burgdorferi.
Marques AR, Hornung RL, Dally L, Philipp MT.
Clinical Studies Unit, Laboratory of Clinical Infectious Diseases,
National Institute of Allergy and Infectious Diseases, National
Institutes of Health, Building 10, Room 11N228, 10 Center Drive MSC
1888, Bethesda, MD 20892-1888. amarques@xxxxxxxxxxxxxx
The Borrelia burgdorferi-specific immune complex (IC) test, which uses
polyethylene glycol (PEG) precipitation to isolate ICs from serum, has
been used as a research test in the laboratory diagnosis of early Lyme
disease (LD) and has been proposed as a marker of active infection. We
examined whether B. burgdorferi-specific antibodies were present within
PEG-precipitated ICs (PEG-ICs) in patients with LD, posttreatment Lyme
disease syndrome, and controls, including individuals who received the
outer surface protein A (OspA) vaccine. Using a B. burgdorferi
whole-cell enzyme-linked immunosorbent assay (ELISA), we obtained
positive PEG-IC results not only in patients with a history of LD, but
also in individuals vaccinated with OspA vaccine. The frequency of
positive PEG-IC ELISAs in OspA vaccinees was significantly higher with
ELISA-reactive than with ELISA-negative unprocessed serum samples (P =
0.001), demonstrating dependency between the tests. Similar results
were found using samples from rhesus macaques infected with B.
burgdorferi, uninfected macaques vaccinated with OspA, and controls.
Therefore, testing for the presence of antibodies against B.
burgdorferi in PEG-IC preparations is not more likely to reflect active
infection than testing in unprocessed serum and should not be used in
individuals who received the OspA vaccine.
PMID: 16148168 [PubMed - in process]
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