TULANE STUDY RESULTS...5 YEARS AND COUNTING...

Grant Number: 1R01AI042352-01
PI Name: PHILIPP, MARIO T.
PI Email: philipp@xxxxxxxxxxxxxxxxxxxxx
PI Title: PROFESSOR AND CHAIRMAN
Project Title: ANTIBIOTIC OF CHRONIC LYME DISEASE IN MONKEYS

Abstract: DESCRIPTION (Adapted from the applicant's abstract): This
project, presented by an outstanding group of investigators that
includes Drs. M. T. Philipp as P.I. and M. S. Klempner, is designed to
complement and expand a human study already in course directed to probe
the efficacy of an antibiotic regimen designed to treat chronic Lyme
disease. The investigators propose to study in rhesus monkeys the
efficacy of ceftriaxone and doxycycline to eliminate B. burgdorferi for
tissues, especially from the central nervous system. The protocol is
two-tiered. In the first tier, 6 monkeys will be tick infected with
neurotropic B. burgdorferi NT1 to confirm the neurotropism of the
strain. One monkey will be left uninfected, as a control. In the second
tier, 24 animals will be tick infected with strain NT1, with four
remaining uninfected. The animals will be divided into two groups (12 +
2) and one will be treated with antibiotics and the other given
placebo. The ability of the antibiotic to clear up tissues from
infection with B. burgdorferi NT1 will be ascertained by a variety of
serially applied procedures that will include: culture and PCR of skin
biopsies; PCR and RT-PCR applied to CSF; immunological detection of
bacterial antigens in urine, and qualitated and quantitated analysis of
anti-borrelial antibodies paired sera and CSF, anti p39 (BmpA) antibody
in serum and levels of matrix metalloproteinases in samples from
nervous tissues. The analysis will also include postmortem histological
examination, culture and PCR studies in tissues from many organs.

Thesaurus Terms:
Lyme disease, antibiotic, chronic disease /disorder, microorganism
disease chemotherapy, nonhuman therapy evaluation
antibacterial antibody, bacterial antigen, enzyme activity,
metalloendopeptidase
Macaca mulatta, microorganism culture, polymerase chain reaction

Institution: TULANE UNIVERSITY OF LOUISIANA
6823 ST CHARLES AVE
NEW ORLEANS, LA 70118
Fiscal Year: 1997
Department: NONE
Project Start: 30-SEP-1997
Project End: 31-AUG-2000
ICD: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
IRG: BM

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