Re: Phillips/Mattman culture medium
- From: spiralgerm13@xxxxxxx
- Date: 31 Mar 2006 09:47:54 -0800
It is so easy to see spirochetes, scientists since Balfour in 1906 have
been watching them and their blebs and cyst forms.
Why have the academics in the last 40 years found it so difficult.
Reading the journal of parasitology recently I see that from 1992
until1999, researchers were taking blood samples in Thailand and Burma
out in the remote villages to check for different malarial parasites.
They put one drop of blood onto an ordinary microscope slide, added a
little Acridine Orange dye, made up in tap water, and within one minute
of looking, they could see their malarial parasites easily, "along with
microfilarial worms and borrelia".
Scientists at the Pasteur Institute in France have published a quite
straightforward technique to find spirochetes in human blood, whether
they are from Syphilis or Borreliosis patients. They specify the
magnification and the number of fields of view (250) that should be
examined before ruling out that there are no spiros present.One
spirochete in a field of view is recognised as being a low level of
infection, but still evidence of a spiralaemia, because 1 spirochete
can release a lot of blebs.
Culturing is more difficult, but adding rabbit serum to BSK seems to
do the trick, according to some labs, so the original BSK might not
have been very good for spiros with outer antigens adapted to human
plasma
...
Culturing seems to be the only gold standard.
But why go to all that trouble when you have a sick person and within a
few minutes you can check for spirochetes?
My spirochetes had been growing and thriving in my blood for decades,
but I didn't see them until last year, under dark field microscopy. The
blood slide had sat in the fridge for 2 days, but the spiros were very
alive.
I've watched one long spiro release a bleb before my own eyes, it's
fascinating.
This post was meant to reply to Lymerager's discussion on microscopy,
hope the same folk read it
Spiral13
.
- References:
- Phillips/Mattman culture medium
- From: LymeRayja
- Phillips/Mattman culture medium
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