Anaplasma phagocytophilum infection of the
- From: "georgia" <jwissmille@xxxxxxx>
- Date: 16 Apr 2006 13:40:12 -0700
"...It is possible that the primary site of infection in HGE is the
peripheral mature granulocytic population......."
In vivo and in vitro studies on Anaplasma phagocytophilum infection of
the
myeloid cells of a patient with chronic myelogenous leukaemia and human
granulocytic ehrlichiosis.
J Clin Pathol. 2004; 57(5):499-503 (ISSN: 0021-9746)
Bayard-Mc Neeley M ; Bansal A ; Chowdhury I ; Girao G ; Small CB ;
Seiter K ;
Nelson J ; Liveris D ; Schwartz I ; Mc Neeley DF ; Wormser GP ;
Aguero-Rosenfeld ME
Department of Pathology, New York Medical College, Westchester Medical
Center, Valhalla, New York 10595, USA.
AIMS: The occurrence of human granulocytic ehrlichiosis (HGE) in a
patient
with chronic myelogenous leukaemia (CML) provided an opportunity to
study
whether Anaplasma phagocytophilum, the aetiological agent of HGE,
infects mature or
immature cells, both in vivo and in vitro. METHODS: Diagnosis of HGE
was
confirmed by culture, polymerase chain reaction (PCR), detection of
intragranulocytic inclusions, and serology. The infection rates of
different myelogenous
stages of granulocytic differentiation were determined by microscopy.
Anaplasma
phagocytophilum infection of the bone marrow was analysed by PCR,
culture, and
microscopy. In addition, the in vitro growth of A phagocytophilum in
the
patient's granulocytes and in HL-60 cells (a promyelocytic leukaemia
cell line) was
compared. RESULTS: Pretreatment blood smears showed that mature
granulocytic
cells had a higher infection rate with A phagocytophilum than did
immature
cells. In the original inoculation of the patient's cells into HL-60
cells to
isolate A phagocytophilum, the bacterium grew faster in the patient's
leukaemic
cells than in HL-60 cells. Anaplasma phagocytophilum inclusions were
rarely seen
in bone marrow granulocytes and PCR was negative. In vitro, two A
phagocytophilum isolates grew faster in the patient's granulocytes than
in HL-60 cells.
CONCLUSIONS: The superior growth in CML cells compared with HL-60 cells
suggests that A phagocytophilum preferentially infects mature
granulocytes. The
higher infection rate of the patient's mature versus immature
granulocytes before
treatment and the minimal level of infection of the patient's bone
marrow
support this. It is possible that the primary site of infection in HGE
is the
peripheral mature granulocytic population.
PreMedline Identifier: 15113857
.
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