oxidation model

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Date: 02/27/05 

Date: 27 Feb 2005 12:09:19 -0800

J Agric Food Chem. 2004 Nov 3;52(22):6818-23. Related Articles, Links

Novel low-density lipoprotein (LDL) oxidation model: antioxidant
capacity for the inhibition of LDL oxidation.

Chu YF, Liu RH.

Department of Food Science and Institute of Comparative and
Environmental Toxicology, Cornell University, Ithaca, New York 14853,
USA.

A novel model of peroxyl radical initiated low-density lipoprotein
(LDL) oxidation (LDL oxidation model for antioxidant capacity, or
LOMAC) was developed to assess the free radical scavenging capacity of
antioxidants and the extracts of natural products. A water-soluble free
radical initiator, 2,2'-azobis(amidinopropane) dihydrochloride, was
used at physiological temperature (37 degrees C) to generate peroxyl
radicals to catalyze lipid oxidation of LDL isolated from human plasma
samples. Headspace hexanal, a major decomposition product of LDL
oxidation, was measured by a headspace gas chromatograph as an
indicator of antioxidant capacity of different concentrations of pure
antioxidants (vitamins C and E) and the extracts of natural products
(fresh apple phytochemical extracts). All vitamin C and E and apple
extract concentrations tested resulted in increasing partial
suppression and delay of LDL oxidation. On the basis of the median
effective dose (EC(50)) calculated for each compound or extract tested,
the LOMAC value of 100 g of apple against LDL oxidation was equivalent
to 1470 mg of vitamin E or to 402 mg of vitamin C. This study shows
that the LOMAC assay can be routinely used to analyze or screen
antioxidants or phytochemical extracts against LDL oxidation to prevent
cardiovascular disease. The food-specific LOMAC values will be very
useful as a new alternative biomarker for future epidemiological
studies of cardiovascular disease.

PMID: 15506821 [PubMed - indexed for MEDLINE]

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