How to isolate amniotic stem cells from the placenta, at home!

Dear Sci.Med Group,

I am Attila Csordás, a trained molecular biologist and
biotechnologist, and my research topic is mitochondrial and stem cell
biology, tissue engineering, regenerative medicine.

Here is a stem cell isolation protocol for everybody, you might have
been interested in:

http://pimm.wordpress.com/2007/01/23/how-to-isolate-amniotic-stem-cells-from-the-placenta-at-home/

Intro:
The placenta is a very valuable and scarce human tissue, although the
proper recycling of it is not placentophagy, but the isolation of stem
cells from its amnion layer, and storing them for later regenerative
purposes for the whole family. Human amniotic epithelial cells (HAECs)
from the placenta are alternative replacements of human embryonic stem
cells, and have the potential to differentiate to all three germ layers
in vitro. These cells are very close to those earlier and broadly
multipotent amniotic fluid-derived stem cells, which made the big buzz
lately on the web, published by De Coppi, Atala et al. in Nature
Biotechnology. Here I would like to show, although I do not provide any
warranty and can not give any guarantee, that isolating stem cells from
the placenta is not more difficult than making a steak, and with proper
preparation, investment and timing you can do it even at home or in a
rent lab. The process is ethically non-controversial since the placenta
is usually discarded after birth. Today, stem cell therapy is just a
promising possibility, but in the not so distant future, self-aware
citizens may manage their own stem cells, grow them in the garage, and
store them in the fridge. If so, it could be a form of autonomous
medical self-insurance. We are at the dawn of the bioDIY movement
backed by open source science for anybody. I used Make magazine's
Backyard Biology issue as a reference, because it invented the basic
language of bioDIY or home or garage biotech. Here is the algorithm at
the cartoon and below are the detailed, although not self-including
textual protocol. More details will come later, if asked.

Cheers, Attila Csordás.

.

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