Re: Does any body know about new light microscopes ?


"josefmatz" <josefmatz@xxxxxxxx> schrieb im Newsbeitrag
news:456596ce$0$18850$9b4e6d93@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
There must be developments that the Abbe Limit of resolution is knocked
out
with a trick.
Resulutions now possible below Abbes limit. Does anybody know more ?


Btw.,
In several magazines lately was published a paper from Betzig and Hess,
where they describe a microscopy which beats the diffraction limit.
Read it for instance here
http://www.photonics.com/content/news/2006/August/11/83891.aspx .
They use a technique to switch on the fluorescence in dye molecules and
switch on only a few of them at a time. So they see the fluorescence of
scarcely distributed molecules as wide blobs with the resolution that the
microscope allows. Then they use some kind of deconvolution to find the
center of the blob and the supposed position of the molecule. After having
determined the positions of every molecule the whole image is gained by
adding up the results of the deconvolutions.
Now my question is:
Does this procedure really promise to deliver sharper images than
conventional deconvolution of an image with all fluorophores present at
once? The deconvolution is a linear process, so it shouldn't make a
difference if you deconvolve a sum of unsharp blobs or sum up the
deconvolution results of single blobs? Both times it's the same PSF that you
need to optimally increase the resolution.

Alexander



.

Relevant Pages

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  • Re: superresolution
    ... They use a technique to switch on the fluorescence in dye molecules and ... Then they use some kind of deconvolution to find the ... need to optimally increase the resolution. ... This should give much higher resolution than conventional microscopy. ...
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