Re: Randomized block design

From: Anon. (bob.ohara_at_NOSPAM.helsinki.fi)
Date: 10/23/04


Date: Sat, 23 Oct 2004 11:08:42 +0300

Veronique wrote:
> Hello,
>
> I'm a PhD-student and I have some trouble with the experimental
> (cage-experiments) design of my project.
> I use a randomized block design with 4 treatments and a control. For
> each treatment and the control, I use 3 replicates. The treatments
> are:
> - Ulva present/predation present
> - Ulva present/predation absent
> - Ulva absent/predation present
> - Ulva absent/predation absent
> The experiment is conducted during one year and a sample is taken each
> month in every experimental unit.
> Now I want to know how I can analyse the results. I want to detect the
> effect of Ulva present/absent, the effect of predation present/absent
> and the interaction between Ulva present/absent and predation
> present/absent. Time is also importent, so I want to know the
> interaction between time and Ulva present/absent and the interaction
> between time and predation present/absent.
> I hope somebody can help me to find a correct statistical analyse

As nobody has replied, I'll leap in. Firstly, what is the control?
It's not clear to me what it would be.

You have the design set out: a full model will have the interaction
between Time, Ulva and Predation. The analysis will be an ANOVA, or
something like it.

The precise analysis will depend on what the response is: the one thing
you forgot to tell us!

There are a number of good books for biologists on statistical analysis:
  one book that would be relevant would be "Modern Statistics for Life
Scientists" by Alan Grafen and Rosie Hails.

Incidentally, the first thing to do is to plot the data: it usually
gives a good indication about the main trends in the data. Plot things
like the response against time for all cages, with the different
treatments in different colours.

My other advice would be to see if your university has a statistical
consulting service that you could go and see: it's always better if you
can have an in-depth discussion, and show your data.

Bob

-- 
Bob O'Hara
Department of Mathematics and Statistics
P.O. Box 68 (Gustaf Hällströmin katu 2b)
FIN-00014 University of Helsinki
Finland
Telephone: +358-9-191 51479
Mobile: +358 50 599 0540
Fax:  +358-9-191 51400
WWW:  http://www.RNI.Helsinki.FI/~boh/
Journal of Negative Results - EEB: www.jnr-eeb.org


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