Re: Digital Reflex on microscope
From: Aaron (nghy_at_comcast.net)
Date: 07/02/04
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Date: 1 Jul 2004 20:19:13 -0500
Hi Gregor,
I have not used or examined the Coolpix 5400. I think that some
people who tried to use the newer versions of the Coolpix with the new
lens (different from the 4500) and the same techniques that worked
with the 900-4500 series cameras ran into problems. Using the older
techniques requires being in the telephoto range and there is
considerable loss of sharpness at the edges and some distortion of the
aspect ratio.
Since all of the new cameras were intended to work with the same slide
copier, I would imagin that the 5400 could be used also. BUT I DON'T
KNOW FOR SURE.
It was with great trepidation that I purchased the 5000. I bought a
refurbished unit at rock bottom on eBay so I would not have too much
tied up in it. My first efforts were a disaster until I figured out
what would work. I have been keeping a low profile for that reason.
Aaron
On Tue, 29 Jun 2004 23:11:56 GMT, "GTO" <gregor_o@NOSPAMyahoo.com>
wrote:
>Hello Aaron:
>
>I just looked at the Coolpix 5400. Do you think, it is a good replacement
>for the 5000 when used for photomicrography?
>
>Thanks,
>
>Gregor
>
>"Aaron" <nghy@comcast.net> wrote in message
>news:9o01e09nee2vk1dvc1amlrmmcdt1rfnfk3@4ax.com...
>> Hi David,
>> .
>> Thank you for the kind words
>> .
>> > Aaron (a very reliable source) states that he has
>> >used a Coolpix 5000 successfully, so it cannot be an insuperable
>> >problem.
>>
>> I agree with you that the need for larger optics is related to the
>> more extreme zoom ranges. As with any telescope the larger front lens
>> gathers more light. The smaller lenses on the 900-4500 series were
>> limiting with a maximum of f4 in the telephoto modes.
>>
>> The Nikon instructions for the Coolpix 5000 settings are the same as
>> for the earlier series and that was my point. By experimentation I
>> found that using the setting intended for a slide copier attachment.
>> eliminated distortions at the edges and included more of the field of
>> view. I was unhappy with results obtained by their suggested
>> settings..
>>
>> The Nikon (brand) adapter is for C mount systems or 30mm ports. your
>> threaded Zeiss lens will fit the same threads. .
>>
>> The above not withstanding, here is the Nikon url for all microscope
>> instructions and
>>
>>
>http://support.nikontech.com/cgi-bin/nikonusa.cfg/php/enduser/std_adp.php?p_sid=aSKp63fh&p_lva=&p_faqid=259&p_created=1031862141&p_sp=cF9zcmNoPTEmcF9ncmlkc29ydD0mcF9yb3dfY250PTImcF9zZWFyY2hfdGV4dD1taWNyb3Njb3BlJnBfc2VhcmNoX3R5cGU9MyZwX3Byb2RfbHZsMT1_YW55fiZwX3Byb2RfbHZsMj1_YW55fiZwX2NhdF9sdmwxPX5hbnl_JnBfcGFnZT0x&p_li=
>>
>> This if for the Coolpix 5000 specifically.
>>
>> http://www.nikonusa.com/pdf/CP5000_microscope.pdf
>>
>> I aquired Zeiss C-mounts with the corrective optics and mounted these
>> on my trinocluar heads. I use the adapter. which designed by Nikon
>> which they recommend for all the Coolpix lines. I can use either the
>> 990 or the 5000 with this relay..
>>
>> I have uploaded two very large jpg files (5meg each) as examples of
>> images made with the CP5000.. The large files are necessary to
>> appreciate the excellent resolution. As it is, the jpg format has
>> lost some of the original information.
>>
>> The anchovey otolith is a DIC image using a 10X Neofluar objective.
>> Otolithss are mineral structures found in the region near the ears..
>> The rings are studied as indicators of growth loosely similar to the
>> groth rings in trees. This image exhibits the ability of the 5000 to
>> capture subtle gradations of color and shading. This slide is a
>> difficult specimen because the otolith is thicker than a typical
>> cross sections and there is no cover glass. It is cemented to the
>> slide with a clear resin..
>>
>> The US Ai rForce test pattern is a thin metal film deposited on a
>> clear glass slide. The image was taken with a Leica M420 with
>> transmitted light at about 4X. It is not well known but low power
>> objectives present the greatest challenge to high resolution cameras
>> because they possess reattively high NA per unit magnification.
>>
>> The smallest groups are just visable using 10X eyepieces. The 990
>> will not resolve the group 7 test patterns. The last file contains
>> the specifications for theresolution test slide.
>>
>> I will not be leaving these files up for long so if you want to refer
>> to them save them but please do not use them elsewhere. .
>>
>> http://home.comcast.net/~nghy/mywebpages/test/Anchovey_otolith.jpg
>> http://home.comcast.net/~nghy/mywebpages/test/USAF.jpg
>> http://home.comcast.net/~nghy/mywebpages/test/Res_target.jpg
>>
>> Aaron
>
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