Re: Calibration

From: Peter Bovey (prbovey_at_REMOVEblueyonder.co.uk)
Date: 12/29/04 

Date: Wed, 29 Dec 2004 14:15:21 GMT

"Andy Resnick" <axr67@op.cwru.edu> wrote in message
news:cqscpi$9r0$1@eeyore.INS.cwru.edu...
> Peter Bovey wrote:
>
>>Is it possible to buy a scale to put on the slide in order to measure the
>>image? My eyepieces don't look as if they will support a reticle. I
>>suppose the problem with putting a scale on the slide might be that at
>>high magnification the scale and the object won't be in focus at the same
>>time. Can any of you offer any advice on this matter? I'm wanting to
>>identify mosses and it appears that I need to be able to measure the
>>cells.
>>
>>Peter
> Peter,
>
> It's not clear from your post if you need to have a reticle/graticule in
> the eyepiece, or some way of having length information on a camera image.
> For the reticle, you could probably figure out a way of putting one either
> in the eyepiece, or down the cylinder into where the eyepiece slides- in
> that case, you need a known object to calibrate the reticle to give length
> information. Edmund scientific carries a bunch and they are cheap. The
> easiest thing is to call Prior and ask them what size reticle is required
> and where it needs to go.
>
> If, on the other hand, you are acquiring images and need to convert pixels
> into microns (or some such thing), you need an image of a calibrated
> ruling. Rulings can be cheap or expensive, depending on the exactitude
> you need. Again, Edmund carries cheap ones.
>
> --
> Andrew Resnick, Ph.D.
> Department of Physiology and Biophysics
> CWRU School of Medicine
> tanspose 'op' for mail
>
Thanks Andy. I'm not interested in aquiring images at the moment. I'm going
to contact Prior and take it from there.

Peter

Relevant Pages

  • Re: Calibration
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  • Re: Calibration
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