Re: sample preparation for water immersion

From: GTO (gregor_o_at_NOSPAMyahoo.com)
Date: 03/17/05

  • Next message: Aaron: "Re: sample preparation for water immersion" 
    Date: Thu, 17 Mar 2005 02:38:58 GMT

    Do you have a centering telescope? With the help of the centering telescope
    you can very easily find any small air bubble that is trapped between your
    lens and your sample. It's not too difficult to move the water immersion
    lens into the liquid after you focused with a dry lower power lens.
    Remember, do not get the dry lens wet and make sure the lenses are parfocal.

    But just for sake of completion you might want to read [1].

    Gregor

    [1] http://www.microscopy-uk.org.uk/mag/artoct04/pjwater.html

    "Fred Chen" <frederick.chen@intel.com> wrote in message
    news:1111002035.047587.231480@g14g2000cwa.googlegroups.com...
    > Hello all,
    >
    > I was curious whether any sample preparation, specifically related to
    > surface wetting, was necessary prior to water immersion for microscopy.
    > The concern is whether air bubbles can be trapped between the water and
    > the sample surface due to insufficient wetting.
    >
    > For NA>1, this would be prohibitive for imaging, as the air pocket
    > would totally reflect light (air cannot support NA>1), preventing the
    > sample region underneath from being illuminated.
    >
    > I have not done any work in water immersion microscopy myself, but I
    > think this issue could have a strong bearing on lithography (which is
    > where I have more involvement). For a water immersion scenario (which
    > is currently gaining popularity), we would want the water to wet the
    > top layer. It appears generally, however, that this is taken for
    > granted. So I am wondering, is there is any way to avoid getting a
    > nasty surprise?
    >
    > Thanks in advance and regards,
    > Fred
    >

  • Next message: Aaron: "Re: sample preparation for water immersion"

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