Re: Photographing bacteria in a Gram's stain
- From: Gary G <see.signature@bottom>
- Date: Sun, 10 Jul 2005 07:38:21 -0700
On Sun, 10 Jul 2005 10:50:59 GMT, "First of Three of Six"
<firstofthreeofsix@xxxxxxxxxxx> wrote:
>"Scott Coutts" <scott.coutts@xxxxxxxxxxxxxxxxx> wrote in message
>news:uQ6Ae.36633$oJ.18937@xxxxxxxxxxxxxxxxxxxxxxxxxxxxx
>> First of Three of Six wrote:
>>> Hi can anyone share tips and tricks on capturing images of bacteria
>>> stained by Gram's method.
>>> I'm using an Olympus BX51 with a DP70 and capturing the images using
>>> Olysia BioReport.
>>> While I'm finding the process excellent for larger organisms via a 40
>>> objective, when using oil 100 focusing and sharpness have suffered.
>>> Everything looks good on the screen, but then I transfer the image (saved
>>> as tif and usually around 36 MB)
>>
Make sure you have plenty of light. The DP70 is not real sensitive.
What 100X objective are you using? What condenser are you using?
At 1000X mag, to really optimum results, I find that a PlanAPO
objective is needed along with an aplanatic condenser. I use a DP70
and all PlanAPOs on my BX51.
Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
.
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