Re: Photomicrography ... objective questions (and general advice)



Hello, Frances:

Here are my two cents:

1) You may want to reconsider whether to buy the E200 or not should you
ever be interested in mounting a good condenser to your scope. The
E200-F only supports Koehler illumination with its Abbe condenser. It
uses a quirky relay lens to make Koeher illumination work. This relay
lens does not screw onto the high-end condensers. (Don't buy APO's or
FLUOR's without getting a good condenser! That's often the reason why
people can't see a clear difference because they are not also upgrading
their Abbe condenser to at least a good Achromatic condenser. The Abbe
condenser on student scopes are really cheap.)

2) Don't buy a new scope if you budget is below US$3000. You will run
out of money fast. Buy a used scope instead. A budget of US$5000 is
more reasonable if you purchase new equipment (not including the
camera!).

3) The best lens for my kind of bright field work is the Nikon Plan
Fluor lens 40x. With a resolution of 0.75, perfect image flatness and a
great 0.72mm WD, the lens is a real killer. The difference between the
cheaper E-Plan Achros and the Plan Fluors is apparent. Again, don't
worry about buying them new and stick with a good used scope. (E.g. the
Plan Fluor 40x lens costs new around US$900.)

4) Try to get a scope with a 38mm ISO port on the trinoc. For the
E200-F you can get one, which costs you around $800 (new).

5) Look at eBay to learn about prices. Check out dealers in your area.
Ask for demo models.

6) Should you buy the E200-F and should you use it with its Abbe slider
condenser and a darkfield slider (or phase slider), you might realize
that you need to apply a very strong torque setting to keep the table
from drifting.

Anyway, the E200 is ok, if you do not buy expensive lenses for it but
you really really want a new scope. Just use it with its E-Plan lenses.

Gregor

.



Relevant Pages

  • Re: Nikon, Leica, or Zeiss?
    ... Axiostar (the Axiostar and Axiostar Plus are 99.9% the same scope!); ... Similar used parts for older Zeiss models sell ... objective with out the condenser. ... I chose Olympus WHK10x 20L high eye point eyepieces rather than Zeiss ...
    (sci.techniques.microscopy)
  • Re: Advice please for a newbie
    ... Again, I have no digiscoping experience, so ... Is 12x optical zoom ona 10 mp digital camera good enough ... on the spotting scope is not very useful, ... less expensive than a standalone 3000 mm telephoto lens. ...
    (rec.photo.digital)
  • Re: Darkfield Condenser
    ... If that's the scope in question, we can forget about adding Phase or DIC. ... I know that you only refer to biological applications. ... DIC is useless with any cell tissue that is birefringent. ... get a darkfield condenser to look at live cells, ...
    (sci.techniques.microscopy)
  • Re: Accu-scope questions
    ... I was leery about buying a used scope, especially on eBay, as it ... As I said, some images are as sharp as a tack, while others ... I never before used an iris diaphragm for taking pictures, ... Never before used a scope with a condenser that could be moved up ...
    (sci.techniques.microscopy)
  • Re: If these lenses are in good shape, this was one heck of a buy!
    ... that the better quality objectives of this scope suffer under serious issues ... The lens is crap. ... I am convinced that this particular seller ... through something similar when purchasing junk on eBay. ...
    (sci.techniques.microscopy)