Re: Photomicrography ... objective questions (and general advice)
- From: "GTO" <Gregor.Overney@xxxxxxxxx>
- Date: 21 Jul 2005 11:04:24 -0700
I may have sounded a little bit too negative regarding newer scopes. So
here are some more thoughts ;-)
In my last post, I forgot one very important point that actually speaks
for the purchase of a new (or almost new) microscope using the CFI60
optics from Nikon (or a used Nikon scope using CF optics). Since you
are interested to use your future scope for photomicrography, a new
scope with CFI60 lenses (or a used one with CF optics) will save you a
lot of troubles. You do not need a relay lens to compensate fully for
CDM. You should read the following website
http://micro.magnet.fsu.edu/primer/anatomy/aberrations.html. The older
Leitz, Zeiss, Olympus and the very old Nikon scopes require a rather
expensive relay lens to generate a beautiful image on the sensor if you
should decide to use better quality lenses (such a FLUORs or APOs).
People find themselves surprised how much a good setup for
photomicrography will cost for an older scope. Some people recommend
lifting out a standard ocular, which works but causes another problem
with the DSLR, which is known as oversampling. The FOV is then terribly
limited. However, a NFK Olympus relay lens does a better job projecting
a reasonable image onto your CMOS sensor (should you use an older
Olympus scope). In case of Nikon's CF or CFI60 optics, you just project
the intermediate image (or real image) onto the image sensor. You will
get vignetting, which is easily removed via post processing. Depending
on your work flow in your digital darkroom, all this can be automated
using batch processing. You end up with a good photograph that offers 4
to 5 MPixels, which is enough to sample your entire FOV.
So, with your limited budget of US$1500++ you may want to look at the
used Nikon scopes using CF optics. They are finite 160mm scopes that do
not require a clumsy and expensive setup to correct CDM, but the sell
at reasonable prices. A Nikon Labophot is a good frame.
Last but not least, do you already have 300D? If not, you may also
consider looking at the 350D, which comes with MLU and better manual
settings. Dave Walker announced an upcoming article about using a 350D
in Micscape Magazine (see
http://www.microscopy-uk.net/mag/indexmag.html). Last but not least, if
you want to take a look at some stuff I was doing with Nikon gears,
take a look at http://www.geocities.com/gregor_o/microscopy.html).
Good luck,
Gregor
.
- References:
- Prev by Date: Re: Letiz info...
- Next by Date: Microscope mechanical repair help?
- Previous by thread: Re: Photomicrography ... objective questions (and general advice)
- Next by thread: Re: Photomicrography ... objective questions (and general advice)
- Index(es):
Relevant Pages
|
