Darkfield condenser
- From: "justbeats" <steve_beats@xxxxxxxxxxx>
- Date: 12 Sep 2005 02:32:33 -0700
I just got a second-hand Zeiss darkfield condenser. IMHO it's the
best bang for the buck of any accessory I've ever bought for my Zeiss
inverted! For diatom imaging at high mag (and high NA), the improvement
in contrast compared to other techniques (e.g. BF, PC, COL or DIC) is
massive. I'd already tried low NA darkfield with excellent results, but
I didn't expect the hi-NA version to be quite so much better again.
Wow!
It's the one accessory I don't mind having to use immersion oil
for! Good job too as it's wholly non-functional if not oiled to the
back of the slide :-)
Couple of questions...
The condenser is marked "1.2/1.4". Obviously this is the NA, but
why two numbers? Is it related to immersion medium (i.e water/oil or
glycerine/oil in this case)?
I have an old set of prepared slides containing many species of
Coscinodiscus, including some extremely rare varieties and two marked
"new sp?". Unfortunately, the slides are very thick and I can JUST
get the illumination onto the specimen but the condenser is touching
the slide (except for a very thin smear of oil between the two
surfaces). I guess thick slides preclude the use of a hi-NA darkfield
condenser? Shame, as it was those that I most wanted to use this DF
technique on. Any way to solve this limitation?
Cheers
Beats
.
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