Re: Relationship of depth of focus to depth of field?

pbamford:

To understand three-dimensional imagery in microscopy, see

M. Pluta, "Advanced Light Microscopy", Vol 1, pages 349ff, Elsevier, PWN
(1988).

Gregor

PS: You did not yet find all that's available on the net regarding this
subject. If I were you, I would get the book I just mentioned.

<pbamford@xxxxxxxxx> wrote in message
news:1132171798.222135.111390@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
>I have found only one set of equations on the web relating depth of
> focus (dfocus) to depth of field (dfield) and am after a reference from
> a text book. Can anyone recommend a reference and page number? The
> equations are from
>
> http://nsm1.fullerton.edu/~skarl/EM/Microscopy/
>
> and go like this:
>
> dfocus = M * M * R / NA
>
> where M = magnification, R = resolution, NA = numerical aperture
>
> and
>
> R = L * 0.61 / N.A
>
> where L is lamba, i.e. wavelength of light. Also
>
> dfield = L / N.A * N.A
>
> therefore
>
> -----> dfocus = M * M * dfield * 0.61 <------
>


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