Re: Olympus Objectives on a Zeiss Microscope

Hi,

I just run across a paper in which the authors used Olympus water
immersion lens on Ziess Axiovert 200M. Just for your reference.

Anal. Chem. 2006, 78, 3444-3451
Electron Multiplying Charge-Coupled Device Camera Based Fluorescence
Correlation Spectroscopy
Balakrishnan Kannan, Jia Yi Har, Ping Liu, Ichiro Maruyama, Jeak Ling
Ding, and Thorsten Wohland

Satoru

nitschemichael@xxxxxxxxxxxxxx wrote:
Hi all;
I have a Zeiss Axiovert 25 (inverted microscope) and like to use
Olympus objectives, because there are some available that have a
correction for a 2mm glass. Both systems have the same thread size, are
infinite obectives, and have the same Parfocal lenght. There is one
difference in the tube lense focal length, but I'm not shure how that
affects the image. And the other thing is that I'm not sure if the
correction-systems are similar. For our application we don't need a
good chromatic correction because we only have monochromatic light, but
I don't want to have a spherical abberation.
So is there anyone who has experiences with the exchange of objectives?
Thank You!

Michael

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