Re: Blood smear - ID cells

"Art M" <Xarthurruhtra@xxxxxxxxxxxxx> wrote in message
news:VVwWg.27718$rg1.22182@xxxxxxxxxxxxx
Very new to this. Recently bought an old AO 160. I was surprised that I
could get a picture just by holding my digital camera up to the eyepiece.

http://server6.theimagehosting.com/image.php?img=blood_dry_labeled.jpg

Can anyone help me ID some of the cells in that photo? I assume A,B and C
are white blood cells (though I have no idea what type they are). I don't
know what 1 through 6 are (5 - may be an epithelial cell?).

The image is of a dried unstained blood smear with no cover glass. I
assume
the odd shapes of the RBCs are caused by surface tension of the fluid
before
drying up. The inset is wet (though after hours since being prepared) with
cover glass. Is the crenellation of the RBCs normal in that case (I didn't
see many of those when I first looked at the slide).

Any comments to help me along would be appreciated.

Thanks

--Art



It's almost impossible to identify white blood cells unless they are stained
with some kind of Romanovsky stain. In the USA Wright's stain is common for
this type of work, The Europeans seem to preffer the May-Grünwald/Giemsa
protocol..

As the differen types of white cells are identified not only by their
morphological caracteristics but also by their reaction to the stains used,
its almost impossible to identify them beond any doubt given a simple dried,
unstained smear...

If this is your furst picture, it's realy not bad!


.

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