Re: Accu-scope questions


<desertrat7374@xxxxxxxxxxx> wrote in message
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On Oct 23, 5:33 am, "Kevin Cunningham" <sms...@xxxxxxxxxxxxxx> wrote:
<desertrat7...@xxxxxxxxxxx> wrote in
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Thanks Kevin, am sorry to learn that Accu-Scope works this way, and I
should have spent more time searching I guess. However, my budget was
limited and this scope seemed to provide what I wanted for a price I
could afford.

I was (am) leery about buying a used scope, especially on eBay, as it
seems quite a gamble, so opted for a new one.

I think I lucked out, as other than some of the saved camera pics,
everything else works very well (I'm comparing them to the old Bausch &
Lomb workhorse scopes I used to use in college and as an army med lab
tech). The mechanism for focus, stage, nosepiece, condenser and
diaphragm all work very well. They seem sturdy and smooth to operate.
The scope is solid,

I'm particularly pleased with the optics as observing proves very good
images, even with the oil immersion 100X lens. It is only the pics I
take with the camera that seem to vary in sharpness and definition.

I may have lucked out and got one built by a competent Chinese :) as
have no complaint (yet) with the functioning nor the scope itself, only
the camera.

I still think it may be the fault of the software rather than the
camera. As I said, some images are as sharp as a tack, while others
fuzzy.

Some more Qs

1. Is there any software out there, at a reasonable price, that would
work better in capturing and saving images that will work with this
setup?

2. I never before used an iris diaphragm for taking pictures, so maybe
I'm at fault here. On several objects, I stopped down most of the way
to get the depth-of-focus I wanted to get all the detail on 3D
subjects. Does doing this possibly make for less sharp pics? I'll go
now and try same thing with it more opened to see.

3. Never before used a scope with a condenser that could be moved up
and down. For most, I've kept it as close to the stage as possible, as
the lighting seemed OK. Would changing that affect sharpness? I doubt
it, but...

If anybody can help with these questions, will appreciate it and
meanwhile, will have to learn to live with this instrument as long as
it does not collapse on me!


Good points. I didn't propose sending you to e-bay, I am *very* familiar
with e-bay problems thats why I recommended dealers that sold used. Spectra
Services is a company I trust and recommend to customers, they haven't let
me down yet, there are a bunch of dealers like them out there.

As to programs I'm mostly familiar with Photoshop. Thats a bit pricey for
you. Most of my customers work at universities, factories and hospitals and
get Photoshop at quite low prices and they need it anyway. I know there are
some similar products out there, google Photoshop.

Your last two questions involve Kohler (second scientist at Carl Zeiss)
illumination. Set up Kohler by turning on the microscope and put a slide on
the stage, focus at low power, switch to 40X and focus then go back to low
power and correct any focus difference with the eyepiece controls. Then
close down the field diaghragm and center it with the two screws located on
the condenser mount. Open the field diaghragm. Now adjust the condenser
diaphragm so that it is a bit lower than the diaphragm of the objective and
look at the image, you may need to lower it a bit more, depends on the
specimen. The condenser diaphragm controls contrast.

Give this a go and let us know how you did.

Kevin Cunningham
SMS



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