Re: Bayer filter and image scale calculations...
- From: "GTO" <gregor_o@xxxxxxxxxxxxxxx>
- Date: Wed, 15 Nov 2006 22:14:32 GMT
This subject has been discussed here many times. Another place where this
subject has been rigorously discussed is the Yahoo group microscope. After
reading all these threads, it becomes perfectly obvious that it will depend
on several factors. But multiplying the physical pixel size by 1.414 offers
a very good estimate for the "so called" effective pixel size.
Camera Physical Pixel Size Effective Pixel Size
Coolpix 990 3.5um around 5um
D200 5.8um around 8.3um
D70 7.7um around 10.9um
However, when using a green interference filter, the physical pixel size is
roughly identical to the effective pixel size.
BTW, one of the best setups for photomicrography, which also works fine for
flash-light photomicrography, is a 1/2" low noise, cooled CCD with 5.2
MPixels, Bayer filter and a 0.5x relay lens mounted on a newer Olympus or
Nikon scope (or any other scope that does not require a relay lens to
correct for CDM). I tested many different configurations on all kinds of
scopes. But by far, the best results are obtained with a dedicated camera
for photomicrography. The ones with Bayer fitlers are very suitable for
flash-light illumination when using color.
Cheers,
Gregor
"justbeats" <steve_beats@xxxxxxxxxxx> wrote in message
news:1163410196.058076.103300@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Photomicrography - direct projection onto color CCD (DSLR)...
I understand the principles in matching image scale to pixel size but I
want to ensure optimum sampling in each of the R, G and B channels, not
just in the underlying (monochrome) array. How does the presence of a
Bayer filter affect the formulae for optimum image scale? Simply
multiply by sqrt(2)?
I found it a tough one to Google for...
Ta.
Beats
.
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