Re: biofilm

Thanks for your response.

Depth of field would be nice, but I think a bigger problem will be
penetrating the biofilm itself. We can probably get the biofilm quite
close to the cover slip with enough engineering.

I am mainly trying to look at bacteria growing close to an opaque
substrate within a biofilm verses those far away from it (will use
fluorescence)


justbeats wrote:
What problem are you trying to overcome? Depth of field...?

Nomarski DIC is very good at isolating specific focal planes in thick
specimens - perhaps this would offer a solution?

Cheers
beats



aorloff@xxxxxxxxxxxxxxxxxx wrote:
Hi all,

Does anyone have information to share about what sorts of microscopes
work or do not work for imaging a approx. 40 um thick fairly dense
bacterial biofilm. We have looked at a deltavision deconvolution
microscope and will also look at a leica confocal.... and there are
probably other confocal microscopes we should look into as well...

Sucess or failure stories with what microscope would be appreciated.

Thanks,

Amber

.