Re: Zeiss phase condenser

Amazing, never seen before. I know of phase condensers for fluorescence
which let short wavelength light (UV) through, but not this. This
sounds like it would 'dilute' the phase contrast effect, which is quite
handy as the phase contrast often is too strong.

Just a thought: try a polarizer under the condenser. It *might* give a
variable blocking of light, although I would expect clear
(non-diffused) light going through the condenser instead of
translucency/opacity.

As far as I am aware, negative and positive phase contrast is done in
the objective, not by the condenser.

René.

Oncologists schreef:

rene wrote:
Never heard of before. So if you're looking in the scope without
eyepiece but with the phase stops in the condenser, what do you see?
1 semi-opaque background with clear ring, 1 completely opaque
background with clear ring


Oncologists schreef:

Yes, the ring is visible
rene wrote:
Do you actually see a ring in the translucent ones??

Oncologists wrote:
justbeats wrote:
I think what you have there is a set of 2 positive phase plates (the
translucent ones) and a set of 2 negative (the opaque ones). Look in

http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html

for more info...

Cheers
Beats
Oncologists wrote:
Can anyone helop with advice about this condenser, please? It is CZ
with the serial number 4337802.
there is an open position, marked J, a darkfield stop , marked D, and 4
phase rings, marked 2, 3,2,3.
These differ in that two are translucent, and two are reflective.
Can anyone tell me what these different types of phase stop are for,
please? I assume that one pair is for normal phase, but the other?
Thanks for any information/advice/experience. I haven't got any phase
objectives to try it with yet, so am anxious to get suitable ones for
this condenser!

Many thanks to all for very helpful advice, hugo j

.

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