Re: microscope condensers



steve schrieb:
I am trying to design a condenser to be used with my Zeiss Axioskop to
achieve Nomarsky optics (DIC). The problem with using the scope "off
the shelf" is that I desire to look at tissue in a living fish.

I have never heard of such a queer task. If you like to look into (!)
the tissue, to see wether a parasite has occupied the fish, you need a
tissue in the "thickness" of app. 20/1000 mm.

The distance between the surface of your object and the surface of the
front lens of the objective ist app. 0.5 to 0.10 mm. And you always
need a cover glass of more or less exactly 0.17 mm between the object
and the objective.

If you think of looking ONto the surface of the skin with illumination
from above, with special objectives without cover-glass, you will only
be able to see the surface of the surface. But you will not be able to
see any parasites WITHIN the skin.

The
ordinary condenser is just too big to insert under the tissue in
question. I have been contemplating using a light pipe for the light
source component of the condenser with a 90 degree mirror at its end. I
was planning to stack a Walleston

Wollaston


prism, polarizer, and top lens on the
mirror. However, it occurred to me that if I could employ the Walleston
and polarizer at the other end of the fiber where the light enters,
then the front profile of the light pipe would remain small. Further,
if the mirror end of the fiber was touching the tissue, the need for an
na. matching condenser top lens might be minimized. Do you know of any
fibers that would maintain the polarization and Walleston-light beam
split from the input to the output end? I am planning to use water
immersion 40 and 63X DIC lenses for the objectives. Thanks , smh
.

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