Re: microscope condensers
- From: "justbeats" <steve_beats@xxxxxxxxxxx>
- Date: 1 Dec 2006 08:04:01 -0800
Are you hoping Nomarski DIC can perform "optical sectioning" on
something the thickness of a fish ;-) It only works over a few tens of
microns at best, and then only in relatively transparent materials.
steve wrote:
I am trying to design a condenser to be used with my Zeiss Axioskop to
achieve Nomarsky optics (DIC). The problem with using the scope "off
the shelf" is that I desire to look at tissue in a living fish. The
ordinary condenser is just too big to insert under the tissue in
question. I have been contemplating using a light pipe for the light
source component of the condenser with a 90 degree mirror at its end. I
was planning to stack a Walleston prism, polarizer, and top lens on the
mirror. However, it occurred to me that if I could employ the Walleston
and polarizer at the other end of the fiber where the light enters,
then the front profile of the light pipe would remain small. Further,
if the mirror end of the fiber was touching the tissue, the need for an
na. matching condenser top lens might be minimized. Do you know of any
fibers that would maintain the polarization and Walleston-light beam
split from the input to the output end? I am planning to use water
immersion 40 and 63X DIC lenses for the objectives. Thanks , smh
.
- References:
- microscope condensers
- From: steve
- microscope condensers
- Prev by Date: Re: Moving from Used to New...
- Next by Date: Re: microscope condensers
- Previous by thread: Re: microscope condensers
- Next by thread: Re: microscope condensers
- Index(es):
Relevant Pages
|
Loading
