Re: More on A.pellucida
- From: Gary G <see.signature@bottom>
- Date: Sun, 28 Jan 2007 11:11:02 -0800
I believe that I have found a definitive reason why A. pellucida is so
difficult to image using LM. When I looked at the inside (concave)
side of the diatom, the pores are about 100nm x 145nm at a pitch of
285nm. With deep UV, one should be able to resolve one pore from
another. However, resolving the pore itself would not be possible.
Now, with new specimens from Klaus Kemp of the outside (convex) of
diatoms, I find new info. These pores are about 30nm x 100nm and are
90 degrees rotated from the inside pores. Basically, the pores look
like:
http://www.photoweb.net/micro/Apellucida-pore.pdf
Thus, if one was trying to see the large pores, the light is
restricted by the underlying small pores. If one was trying to
resolve the small pores, that already is a no go.
http://www.photoweb.net/micro/Apellucida-100kx-1.jpg
already showed this. Look at the lower right area and one can see the
90 degree difference of inside and outside pores.
gg
Kiss French. Drink California.
.
- References:
- More on A.pellucida
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