Re: Darkfield Condenser


<mythirdeyeisopen@xxxxxxxxx> wrote in message
news:1174885352.307049.58750@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
I recently bought an M Series microscope (Model BM-100FL). I want to
get a darkfield condenser to look at live cells, but I'm having
trouble determining which would be the correct one to buy. The
specifications provided in my manual indicate:

Condenser - Abbe N.A. 1.25

I'd like to buy a cheap one off ebay if possible. Can I buy a
condenser rated "D 1.20A" ? Are all condensers the same diameter? How
do I know if it will fit my scope, and lastly, how difficult is it to
install?

I really appreciate any answers you can give me.

Thanks,
- Dan


You've made just about every mistake you could have. Phase contrast or
Differential Interefenece Contrast (DIC) are the standards for researcher.
These techniques fully support live cells. I've been in the microscope
industry since the late '70's and I've sold one dry dark field condenser.

Now the first question would be what microscope is an M? We need to know
the maker. Next do you have a diaphragm or a stop with your 100X? If not
it doesn't matter which condenser you get the 100X won't work. You can't
get high resolution with dark field since you have to reduce the NA of the
objective. If you want to use an oil dark field then it will say "oil dark
field". The type of condenser you get will be based on the kind of
microscope you have, different types of mounts are used by different makers.
There is an old fashioned type that is kinda universal but it only applies
to some student microscopes.

Thanks, let us hear back from you.

Kevin Cunningham
SMS




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Relevant Pages

  • MICROSCOPY ADVICE
    ... My Biological Microscope Model SB-BI-1 ... Tube: 30 degree, ... adjustment 55-75 mm on both tubes. ... Condenser: Abbe type N.A. 1.25 condenser with iris diaphragm, ...
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    ... My Biological Microscope Model SB-BI-1 ... Tube: 30 degree, ... Condenser: Abbe type N.A. 1.25 condenser with iris diaphragm, ... DARK FIELD: Also they offer me the following: ...
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  • Re: Darkfield Condenser
    ... get a darkfield condenser to look at live cells, ... These techniques fully support live cells. ... Now the first question would be what microscope is an M? ...
    (sci.techniques.microscopy)
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