Re: variable phase contrast


Hi everyone. Sorry to come into this conversation so late, especially
when I don't have too much to add.

I have a CZJ Phv phase set, and a single CZJ older "Ph" 10x objective.
So, this is only confirmed on a 10x objective, but my guess is that
there is a good chance it'd scale up the same through objectives of
different magnification.

As the Phv objectives have two different sets of phase rings/annuli
within them, I kind of would make sense that the Ph (single phase ring)
objectives would be a match to one of the sets of the Phv system.

I tried the objective I have with the Phv condenser on the "10x" annuli
and the ring did match up perfectly to one of the rings of light
produced by the condenser. Unfortunely it was the larger outside ring
it corresponded to. Had it been the smaller, then the larger ring could
be masked off with the condenser's iris diaphragm, but that wasn't the
one they chose.

This means that there was still the smaller inside ring that was
producing a normal brightfield image, and therefore there was no
reasonable phase contrast image produced.

This could fixed either on a temporary or permenant basis by masking off
the inside ring of the annuli within the condenser. The annuli are
printed onto glasses plates in the condenser, but only on one side.
Therefore almost anything can be done to the other side of the glass
plate (painting, taping, etc.) without any risk to damaging the pre-
existing annuli since it is on the other side.

Lightly fixing an opaque mask that fits inside the large ring, but masks
off the smaller ring with a dab of vasoline would probably make the Ph
objective/Phv condenser combination work perfectly.

Hmm..

However, this far into my response, I now realize that it was the
opposite that was asked (Phv objective/Ph condenser). Since at least
one of the annuli match up, my guess is that there shouldn't be any
major problem. This situation is way either actually, as there is only
one cone of undeviated light which should all traverse through one of
the neutral density rings within the Phv objective. Technically some of
the deviated light will be retarded slightly less out of phase by
travelling through the neutral density ring, rather than the phase ring
in the objective, I suspect the effect of this will be minor and likely
unnoticable.

Unfortunetly I can't test this, but since it happens routinely in the
normal Phv system when the iris is closed down to only use the central
annuli then it must be alright.



I hope that helps,

Kevin


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"rene" <renevanwezel@xxxxxxxxxxx> wrote in news:1175805207.835797.267350
@q75g2000hsh.googlegroups.com:

But whether Zeiss (aus) Jena Phv will work for Zeiss West Ph is an
open question.

René.

On 5 Apr, 14:07, "Perl" <thl4...@xxxxxxxxx> wrote:
Hi !

I guess Zeiss Ph Condenser will work with Zeiss Ph Objectives

Regards
Pär



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