Re: New microscope for DIC brightfield and darkfield imaging

The 5Mpix makes sense only at low magnification. Part of our work is
documentation and quality control, where lower magnification (5x, 10x,
20x) will give a trade-off between spatial resolution and larger field
of view. For the details we use 100x objectives, but then we don't
need the 5mpix. However we don't want more than one camera on the
microscope.

Does anyone has comments on the objectives? Olympus use plan
fluorites. Nikon does not use this term anymore, but claims that the
optical performance is the same and that they have moved away from the
term "fluorite" after they changed some processes? Nikon claims that
their objectives are better than the Olympus plan fluorites.


On Apr 17, 10:29 am, heini <buerg...@xxxxxxxxxxxxxxxxxxxxxxxxxxxxx>
wrote:
Hi,
I am not familiar with color photography in microscopy.. as a
fluorescence microscopy user I alwas use B/W cameras.
So first question: what magnifications do you use? 5 Mpxis far too
much if you want to go real close. Good, that these cameras do have
binning at least. I posted a calculation explaining why more than 2
mpx make hardly sense in light microscopy in this forum before.
(considering you are using objectives higher than ~40x)
Maybe check out Pixelink, too.

yours, Heinrich

Cameras: CIII (Olympus AnalySIS) or Qimaging micropublisher (ImagePro/
Nikon)


.

Relevant Pages

  • Re: magnification of 250
    ... the total magnification of the microscope. ... In theory there are 1.6 objectives out there built by Carl Zeiss, ...
    (sci.techniques.microscopy)
  • Re: How many pixels?
    ... needed for some common objectives.. ... of the microscope onto the CCD increases that demand. ... the NA per unit magnification of various objectives that I own. ... approximate number of resolved pixels in the exit pupil as 0.64 Megapixels. ...
    (sci.techniques.microscopy)
  • Re: magnification of 250
    ... the total magnification of the microscope. ... giving a mag of 1600x with a 1x tube and 100x objective). ... use a 40X objective and 20X eyepieces, details get more blurred than when I ... the existence of misterious 120X objectives, I was so excited by the news ...
    (sci.techniques.microscopy)
  • Re: magnification of 250
    ... use aplanatic condenser with double oil. ... So magnifications above 1000x are useless, empty magnification, ... every one selling 60X objectives sells 40X objectives. ... favorably with SEM photographs of the same objects. ...
    (sci.techniques.microscopy)
  • Re: How many pixels?
    ... as to how many pixels are needed to take advantage of the resolution ... of a microscope objective lens when using a CCD camera as detector. ... Only one of the four objectives of more than 40x exceeds a megapixel ... are objectives which offer high numerical apertures for their magnification, ...
    (sci.techniques.microscopy)
Loading