Re: New microscope for DIC brightfield and darkfield imaging

On Apr 17, 7:45 am, "Kevin Cunningham" <sms...@xxxxxxxxxxxxxx> wrote:
<runa...@xxxxxxxxx> wrote in message

news:1176799769.157731.306830@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

The 5Mpix makes sense only at low magnification. Part of our work is
documentation and quality control, where lower magnification (5x, 10x,
20x) will give a trade-off between spatial resolution and larger field
of view. For the details we use 100x objectives, but then we don't
need the 5mpix. However we don't want more than one camera on the
microscope.

Does anyone has comments on the objectives? Olympus use plan
fluorites. Nikon does not use this term anymore, but claims that the
optical performance is the same and that they have moved away from the
term "fluorite" after they changed some processes? Nikon claims that
their objectives are better than the Olympus plan fluorites.

Nikon and everyone else has switched from native flourite to man made
imitations. The imitations have all sorts of advantages, the biggest one is
the different types. I've seen the objectives your talking about and I like
them. A lot.

Another consideration is my experience with Olympus Industrial is unbroken
failure. The Olympus is a good instrument but the service is awful, just
hiddeous. Now, I only know Olympus Industrial in America and Europe, other
places they might be fine. Now Olympus bio is just fine.

Thanks,

Kevin Cunningham
SMS





On Apr 17, 10:29 am, heini <buerg...@xxxxxxxxxxxxxxxxxxxxxxxxxxxxx>
wrote:
Hi,
I am not familiar with color photography in microscopy.. as a
fluorescence microscopy user I alwas use B/W cameras.
So first question: what magnifications do you use? 5 Mpxis far too
much if you want to go real close. Good, that these cameras do have
binning at least. I posted a calculation explaining why more than 2
mpx make hardly sense in light microscopy in this forum before.
(considering you are using objectives higher than ~40x)
Maybe check out Pixelink, too.

yours, Heinrich

Cameras: CIII (Olympus AnalySIS) orQimagingmicropublisher (ImagePro/
Nikon)- Hide quoted text -

- Show quoted text -

Megapixels and pixels size should not be confused. QImaging has a 4 MP
camera with 7.4 micron pixels and 3/5 Mpixel cameras with 3.4 micron
pixels (as does almost everyone). And then it depends upon the coupler
magnification as to what the projected pixel size really is.

David Barnes/ QImaging

.

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