Re: New microscope for DIC brightfield and darkfield imaging

I will be working mostly on metals, semiconductor and interface
between metal/semiconductor and packaging.
My colleagues will be working on optical coatings and surfaces of
optical crystals and mirrors.

Due to a clueless salesperson Zeiss was left out very early in the
process. Later Also Leica fell out of competition parlty due to
organization, but mostly because of the very open design of the two
Japanese competitors. Now that Zeiss is back in the game I have to
check them out. But again, the modular system of Olympus and Nikon
will suit a research lab very well.

- Runar




On 23 Apr, 14:49, "Kevin Cunningham" <sms...@xxxxxxxxxxxxxx> wrote:
<runa...@xxxxxxxxx> wrote in message

news:1177322942.900321.19710@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

Does Nikon have LabView instrument drivers? I have found the driver to
Olympus on ni.com, but not for Nikon.
We have site license for pretty much all software from NI.

Also, suddenly Zeiss wants to sell microscopes. They have now changed
their organization and sells microscopes directly to customers. I
will check their offerings later this week. They claim that they will
be competitive also on the price. Does anyone have comments on the
Axio Imager?

- Runar

Zeiss has always wanted to sell microscopes, just most times they didn't
know how to sell. They used to be no. 2, then then went direct and they
fell to no. 3. They still haven't really got dealers though.

The Axio Imager is a neat 'scope if you can afford it and you do bio. I
expect a few to show up locally (in the SE, USA) in the next year.

Thanks

Kevin Cunningham
SMS

On 18 Apr, 23:39, dbarnes...@xxxxxxxxx wrote:
On Apr 17, 7:45 am, "Kevin Cunningham" <sms...@xxxxxxxxxxxxxx> wrote:

<runa...@xxxxxxxxx> wrote in message

news:1176799769.157731.306830@xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

The 5Mpix makes sense only at low magnification. Part of our work is
documentation and quality control, where lower magnification (5x,
10x,
20x) will give a trade-off between spatial resolution and larger
field
of view. For the details we use 100x objectives, but then we don't
need the 5mpix. However we don't want more than one camera on the
microscope.

Does anyone has comments on the objectives? Olympus use plan
fluorites. Nikon does not use this term anymore, but claims that the
optical performance is the same and that they have moved away from
the
term "fluorite" after they changed some processes? Nikon claims that
their objectives are better than the Olympus plan fluorites.

Nikon and everyone else has switched from native flourite to man made
imitations. The imitations have all sorts of advantages, the biggest
one is
the different types. I've seen the objectives your talking about and I
like
them. A lot.

Another consideration is my experience with Olympus Industrial is
unbroken
failure. The Olympus is a good instrument but the service is awful,
just
hiddeous. Now, I only know Olympus Industrial in America and Europe,
other
places they might be fine. Now Olympus bio is just fine.

Thanks,

Kevin Cunningham
SMS

On Apr 17, 10:29 am, heini <buerg...@xxxxxxxxxxxxxxxxxxxxxxxxxxxxx>
wrote:
Hi,
I am not familiar with color photography in microscopy.. as a
fluorescence microscopy user I alwas use B/W cameras.
So first question: what magnifications do you use? 5 Mpxis far too
much if you want to go real close. Good, that these cameras do have
binning at least. I posted a calculation explaining why more than 2
mpx make hardly sense in light microscopy in this forum before.
(considering you are using objectives higher than ~40x)
Maybe check out Pixelink, too.

yours, Heinrich

Cameras: CIII (Olympus AnalySIS) orQimagingmicropublisher
(ImagePro/
Nikon)- Hide quoted text -

- Show quoted text -

Megapixels and pixels size should not be confused. QImaging has a 4 MP
camera with 7.4 micron pixels and 3/5 Mpixel cameras with 3.4 micron
pixels (as does almost everyone). And then it depends upon the coupler
magnification as to what the projected pixel size really is.

David Barnes/ QImaging


.

Relevant Pages

  • Re: New microscope for DIC brightfield and darkfield imaging
    ... documentation and quality control, where lower magnification (5x, 10x, ... For the details we use 100x objectives, ... their objectives are better than the Olympus plan fluorites. ... I am not familiar with color photography in microscopy.. ...
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