Determining refractive index of silicate microspheres
- From: s.engelhardt@xxxxxxxxxxxxx
- Date: Mon, 02 Jul 2007 05:03:26 -0700
Hallo evereybody,
for a project, I have to use silicate microspheres (10 micrometer) in
a liquid solution with the same refractive index as that of the
spheres. I tried phase contrast microscopy, but the expected shift
between dark and bright inside the spheres when the refractive index
of the liquid is lower or higher can't be seen. It looks more, like
there is brightness gradint insede the spheres that varie with the
refractive index of the liquid. Is this effect due to the spheres
curvature?
As another attemped i tried finding a light scattering minimum with
darkfield microscopy. But, there was now minimum. The intensity just
decreases with increasing refractive index of the sphere...
Has anyone an idea why those experiments didn't work??
thank you for your answers....
sascha
.
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