Re: Photomicography with 2.5x objective - uniform illumination?



On Mon, 16 Jul 2007 10:18:47 -0700, selwyn.stleger@xxxxxxxxx wrote:

I manage reasonable photos using medium and high power objectives but
have been having difficulty with a Zeiss plan 2.5 x objective.
Visually, the image is stunning and evenly illuminated. However,
photography is another matter: there is severe vignetting and obvious
non-uniform illumination from a central bright spot outwards.

I am using a Zeiss standard 16 with inko condenser. I have removed the
top condenser (1.4 NA) lens which I am led to believe leaves a circa
0.32 NA condenser lens. I set up for Kohler as best the system will
allow. The sub-condenser switchable lens is left in place.

Do I need a condenser of lower aperture?

Do I need a diffusing filter?

Or what?

I should be grateful for suggestions.

Selwyn

This happens with all scopes I have used. The low power objectives
need the "ultra low" condenser. For my Oly BX scopes, it is the only
way to go to get even coverage.

I have a new Zeiss 44 53 12 lens thing that looks like a swing out
ultra low power condenser. This might be what you need.



Kiss French. Drink California.

gary at gaugler dot com
.



Relevant Pages

  • Re: How to setup Zeiss DIC?
    ... A Carl Zeiss standard microscope, several objectives (normal and phase ... - what other parts do I need to make the DIC condenser work ... leaving DIC prisms in place for BF and PC work. ...
    (sci.techniques.microscopy)
  • Re: How to setup Zeiss DIC?
    ... A Carl Zeiss standard microscope, several objectives (normal and phase ... what other parts do I need to make the DIC condenser work ... Zeiss" on the top surface) then you need the Zeiss type III slider ...
    (sci.techniques.microscopy)
  • Re: Zeiss objective lens
    ... Zeiss Axioplan 2 scope. ... for used lens. ... For objectives try Spectra Services or Martin ...
    (sci.techniques.microscopy)
  • Re: Zeiss objective lens
    ... Zeiss Axioplan 2 scope. ... for used lens. ... 160mm tube length objectives ...
    (sci.techniques.microscopy)
  • Re: Photomicography with 2.5x objective - uniform illumination?
    ... I am using a Zeiss standard 16 with inko condenser. ... NA condenser lens. ... Using light from a north window in a mirror will give the most even ... If you were using other objectives and lens as I am for macro ...
    (sci.techniques.microscopy)

Loading