Re: New microscope for DIC brightfield and darkfield imaging
- From: shiraz14 <shiraz14@xxxxxxxxxxxxxx>
- Date: Wed, 01 Aug 2007 08:26:06 -0700
On Jun 24, 2:22 pm, shira...@xxxxxxxxxxxxxx wrote:
On May 11, 1:56 pm, "gto" <grego...@xxxxxxxxxxxxxxx> wrote:
"> Well, then the contrarian view would seem to say that this newsgroup> is useless.
...
This does rather poorly summarize what I wrote. I would hardly consider the
information that has transpired here to be in any way conventional wisdom.
This group's input has some value. But it's far from perfect since a few of
its "members" have collected some bad experiences with certain excellent
scopes and do not shy away from discouraging others.
As so often with the Internet, the voice of people with bad experience is
much louder than the one of the satisfied customers.
Cheers,
Gregor
Hi,
I agree ... sometimes its relatively difficult to decide just based on
each sales rep's demo - some features are more often than not not very
essential (e.g. the closed loop linear encoder feedback with 10nm
accuracy employed in the AxioImager - this is not very useful if you
are merely using the scope to do simple applications e.g.
photomicrography, etc since different points of a specimen would
require different Z-positions unless you are thinking of highly
advanced optical sectioning & Z-stack imaging processes) ... after
doing extensive research, I decided to go with a Leica DM4000M scope
with external motorized Z-focus from Prior Scientific and am now
thnking of upgrading it further with filter wheels, etc - the major
advantage is that you have automation when you really want it and if
anything breaks down (e.g. your Z-focus motor which is possibly the
worst case scenario, considering the importance of focussing in both
basic and advanced applications e.g. Z-stacking), you can always
remove the motor & its focus sleeve and revert back to manual Z-focus,
a;lowing you to get some tasks e.g. basic photomicrography of
specimens done quickly and easily. The DM6000 might seem attractive
but there's a catch to it (from what I saw in the local sales rep demo
scope - the Z-focus once broken down cannot be operated manually hence
you'd have to send the whole microscope in for repair ... :| )
The DM4000M I'm currently using allows one to do a range of
techniques, including BF, DF, DIC, Pol, Phase Contrast (in TL) and BF,
DF, Pol, DIC, Fluo (in IL). Fluorescent capability can even be
extended to allow the scope to do multi-FISH applications using filter
wheels from Prior {and 2 quad-band filter cubes from Semrock [(pinkel
or sedat configurations) - which is what I'm considering now ...]}
The optional Pol module or advanced conoscopy module (available for
use with the DM2500P scopes can also be adapted to this scope,
allowing you to do conoscopic analysis (if Pol's your main concern)
and the analyzer be substituted with an iris (if you wish to do
suppression of out-of-focus light rays, should there be a need to),
although you'd have to modify this manually (without technical
support ...) Alternatively, you might choose to go with the DM4500P (I
think this scope's preferable to the DM4000M, but you might need to
obtain the magnification modules from Zeiss (as components of their
AxioImager) in order to achieve higher-than-the-supplied
magniifcations of 1X & 1.6X ... do check this with the filter wheel
diameter first though ... :)- Hide quoted text -
- Show quoted text -
Hi,
Just a note - I've yet to try out the iris mounted in the analyzer
slider - this is just a hypothesis (that it would exclude out-of-focus
light rays from the camera, generating sharper images (which may or
may not be true ... )
.
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