Re: Request for information about Knife Edge Illumination, etc.
- From: UKOncology@xxxxxxx
- Date: Wed, 08 Aug 2007 10:55:53 -0700
On 29 Jul, 13:22, selwyn.stle...@xxxxxxxxx wrote:
On 29 Jul, 13:07, selwyn.stle...@xxxxxxxxx wrote:
On 29 Jul, 02:47, Alan <lngn...@xxxxxxxxx> wrote:
I have plunked about with my inferred version of knife edge
illumination after reading a brief bit about it, somewhere, about 20
years ago. Can anybody offer some insightful tips on variations of
this technique? I have used a card covering about 1/2 of the field
iris of a Zeiss Standard 16, with reasonable results. I suspect with
more insight, a better result can be had. Any ideas would be
appreciated.
My Standard 16 was put together by Zeiss from parts in their shop, at
a discount, many years ago. It is a monocular scope, with a 1.35 na
condenser. The condenser does not have any means available for
insertion of under-condenser filters. I have gotten the correct
screws for several threaded holes on the bottom of the unit. Is it
inadviseable to insert filters underneath?
Interestingly, I have kept this scope in ambient environment
conditions for over 20 years in the tropics, very seldom in an air
conditioned room. Unlike especially an Olympus SZH/10
stereomicroscope purchased over the Internet, the Zeiss has not yet
suffered severe crazing due to fungi (knock on wood). The SZH/10 is a
mess, especially the internal optical train.
Thank you for any suggestions about knife edge illumination.
Alan
Hi Alan,
What you are doing seems similar to Baryy Piekos' Diffracted Light
Contrat.
http://www.kscitech.com/MicGroup/Msg_06/index.htm
http://www.microscopy-uk.org.uk/mag/PiekosArticleMicroscopyTodayNov06...
http://www.patentmonkey.com/PM/patentid/6600598.aspx
http://oe.osa.org/viewmedia.cfm?id=90277&seq=0
Selwyn- Hide quoted text -
- Show quoted text -
Addendum
Piekos' first paper was:
W. BARRY PIEKOS
Diffracted-Light Contrast Enhancement: A Re-Examination
of Oblique Illumination
MICROSCOPY RESEARCH AND TECHNIQUE 46:334-337 (1999)
This does not seem to be freely available for download but I have
emailed you a copy.
Selwyn
Yep, I saw this work too, and have made several attempts with
different edges to try it out.
Not impressed with results at all, but maybe I did'nt get it right.
Cheap enough to try though - I used a modified Stanley knife blade for
my efforts. Hope you have more luck!
.
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