Re: What is a metallurgical objective?


"justbeats" <steve_beats@xxxxxxxxxxx> wrote in message
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On 26 Oct, 00:39, "heliogabalus" <forbid...@xxxxxxxxx> wrote:
"justbeats" <steve_be...@xxxxxxxxxxx> wrote in message

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On 21 Oct, 18:31, UKOncol...@xxxxxxx wrote:
I got nice
closeups of stomata by stacking shots in HeliconFocus.

when i try to assemble the images with helicon the single frames are
dislocated, so the final image presents borders of the images below

can i know the reason of it?

Couple of possibilities...

1. You are not using enough images and your individual focus planes
are too far apart. Take more pictures more closely spaced (in z), that
definitely solves the "stairstep edges" problem - which is most likely
what you're seeing...

2. In the options, ensure the ability to shift the image left and
right during alignment is enabled. However, I always turn off the
option to allow rotation for alignment as that sometimes does the
wrong thing and leaves edge artefacts

Hope that helps...


yes , that helped : )


.

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