Re: Release of Improved Tria Image Processing Program

Well, I slightly disagree...
Image Processing in ImageJ is cool, I love the macro-options for batch-
processing and the measurement-tools, µ-manager....
But good deconvolution of fluorescent images is hard work. One can
easily spend weeks in creating a good PSF for one good stack. One PSF
per color. New PSF if the slice is thicker. New PSF for other camera/
objective/specimen... I have an old IA64 with 2 Processors and 8GB of
RAM running on linux to do the hard work, on normal desktops I always
run out of memory when processing stacks. This is definitvely the poor
man`s solution.
What a difference to paste images into huygens (for example) and have
them deconvoluted without creating PSFs first in less than days! But
Huygens is too expensive for my institute.
Too bad for Tria it won`t run on WinXP64, I'd liked to try it.

Regards, Heini

.

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