Koehler illumination in conoscopic observation



Hello,

On a Nikon scope of type L-Ke the illumination
is truly of the Koehler type. The filament of the
tungsten light is imaged onto the diaphragm of
the condenser lens and is centered and clearly
visible at the back focal plane of the objective
when viewed through a Barlow lens.

Koehler illumination is considered to be an ad-
vantage for most types of observation. It is of
apparent disadvantage when one wishes to ob-
serve the interference figures formed by bire-
fringent crystals using the conoscopic form of
observation in polarization microscopy. In that
case the interference pattern formed by the
crystal is superimposed upon the image of the
lamp's filament causing difficulty in clearly seeing
it..

Does anybody know of a way to get around this
problem? One might think of a diffusing filter
someplace in the path of illumination. If this is
a feasible approach where would one place this
filter and what kind should one use?

Thanks for any suggestions
GR.


use conoscopic observation of the
interference figures formed by birefrigent crystals in the
manner


.



Relevant Pages

  • Re: Koehler illumination in conoscopic observation
    ... is truly of the Koehler type. ... Koehler illumination is considered to be an ad- ... lamp's filament causing difficulty in clearly seeing ... Kohler illumination was done with a lamp who's ...
    (sci.techniques.microscopy)
  • Re: Kohler illumination question...
    ... necessary to get a focussed image of the lamp filament when setting up for Kohler illumination? ... the filament defocussed to varying degrees. ... required - a frosted glass helps a lot in hiding imperfections. ... shape and radiation uneveness of the light source itself. ...
    (sci.techniques.microscopy)
  • Re: Kohler illumination question...
    ... In one of the prior posts you said that "Kohler illumination ... Since the filament is not a flat surface some portions of the filament and its reflection will not be at the same plane and therefore will not be in the same focus further on. ... The easiest way to see if the illumination optics are well-designed is to set the optics for critical illumination and to examine the image of the filament with the objective lens to determine how completely focused the filament is. ...
    (sci.techniques.microscopy)
  • Re: =?windows-1252?Q?K=F6hler_illumination_question=2E=2E=2E?=
    ... If you look at the illumination design of larger research microscopes, especially those which have a relay for illumination then you find these devices implanted there. ... Rene wrote: ... also not in theory (when applied with field iris). ... iris, instead of the filament. ...
    (sci.techniques.microscopy)
  • Re: Kohler illumination question...
    ... necessary to get a focussed image of the lamp filament when setting up for Kohler illumination? ... the filament defocussed to varying degrees. ... Critical illumination, for all practical purposes, is functionally ...
    (sci.techniques.microscopy)