Need help with wavefront distortions on filter surfaces
- From: Optik <mehmet.dogan@xxxxxxxxx>
- Date: Wed, 9 Jul 2008 13:05:21 -0700 (PDT)
Hello,
I have a question and I thought someone in this group might have some
experience with this.
I am trying to setup a confocal fluorescence microscope and would
like to use a dichroic mirror to separate the collected fluorescent
signal into two arms. The transmission arm works OK, however, I don't
get a good focusing on the reflection arm. It turned out that the
dichroic is distorting the wavefronts in reflection so much that I
can't efficiently focus it through the pinhole.
The filter company says the coating on the filter puts stress on the
filter and causes it to bend slightly. I just have the bare filter
without any mount.It's 1mm thick.I tried mounting on metal fixtures
and using rings, gluing the filter to aluminum filter holders, but my
every attempt failed and did not improve focusing. Do you have any
suggestions for mounting the filter so that I make sure that the
filter surface if flat? Should I purchase a thicker dichroic?
Thanks,
Mehmet
.
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