Re: viability assay

On Nov 18, 12:26 am, MariaP <phootos...@xxxxxxxxx> wrote:
I have a Zeiss Imager which I am learning to use. I want to use it for
a viability assay. Will bright field illumination work? Or should I
set up the DIC or phase contrast modes? Any tips/resources on how to
do this? What additional equipment do I need?

In my day, assuming you are looking at epithelial cells in monolayer
culture, we just used phase or old fashioned trypan blue exclusion
with brightfield. No doubt there are much better methods nowadays
involving automation or flow counting. Depends on the specifics of
your application, really.........
.