Implications of no coverslip on objectives corrected for coverslips

From: Ian Shieh <ishieh@xxxxxxxxxxxxxx>
Date: Tue, 03 Feb 2009 15:54:18 -0800

Hi,

I am using a Nikon C1 confocal microscope to image surfactant molecules at an air-water interface. Currently, I use a Plan Apo 20x objective, NA = 0.75, air "immersion" with 0.17 mm coverslip correction. However, when I image the air-water interface, the optical path does not include a coverslip. I have a very limited understanding of optics, but how might not including a coverslip affect spherical/other aberrations? Any insight is greatly appreciated!

-Ian
.

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Re: Implications of no coverslip on objectives corrected for coverslips
... I am using a Nikon C1 confocal microscope to image surfactant molecules ... Currently, I use a Plan Apo 20x objective, ... when I image the air-water interface, the optical path does not include ... might not including a coverslip affect spherical/other aberrations? ...
(sci.techniques.microscopy)
Re: Implications of no coverslip on objectives corrected for coverslips
... I am using a Nikon C1 confocal microscope to image surfactant molecules ... when I image the air-water interface, the optical path does not include ... you will get spherical abberation if the specimen is ... on the surface of the water. ...
(sci.techniques.microscopy)