Re: Banana shaped PSF

Franco Del Principe wrote:
Hello

A customer of our's using a nipkow-disk confocal microscope reported
recently a banana shaped PSF in z (vertical axis). He measured this by
running a X-Z scan of fluorescent beads. In a well-aligned microscope
system a X-Z scan normally produces a cigar-like shape of the PSF. What
is the most probable cause of that cigar shape bending to one side
creating a banana shape? Could it be simple spherical aberration or
rather an oblique lens in the optical path?

Thanks for suggestions.

Maybe this is an explanation for it:
http://support.svi.nl/wiki/Banana-shapedPsfPinholeScanLensImpFaq
http://support.svi.nl/wiki/BananaPsf

DR
.

Quantcast